Starting Integrative Genomics Viewer (IGV) on Rackham/Snowy¶

This guide will go through step by step how to start Integrative Genomics Viewer.

Step 1: Connect to UPPMAX with X-forwarding enabled. (Important step)¶

In a terminal, use SSH with X forwarding enabled:

ssh -X [user name]@rackham.uppmax.uu.se

For example:

ssh -X sven@rackham.uppmax.uu.se

Windows users: we recommend the SSH client MobaXterm
MacOS users: the built-in SSH client ssh does need XQuartz installed too

Step 2: Reserve a node using "interactive"¶

Since genomic sequences require lots of memory, it is not suitable to run IGV on one of the login nodes. That would slow down the response times for all other users on the same login node..

Instead, reserve a node that you will have all by yourself. This command will reserve a whole node for 12 hours, the maximum amount of interactive time you can get and still receive a high priority for your job (feel free to change that if you want to).

interactive -A [UPPMAX project id] -p node -t 12:00:00

For example:

interactive -A snic2017-7-274 -p node -t 12:00:00

For interactive session on Snowy add the flag "-M snowy":

interactive -A snic2017-7-274 -M snowy -p node -t\ 12:00:00

Step 3: Load the IGV module¶

When your job has been started, type the following command to load the IGV module:

module load bioinfo-tools IGV

Step 4: Start IGV¶

To start IGV, type the following:

igv-node

That's it, now IGV should be loaded and ready to go. For more information about how to use IGV, please visit IGV's user guide.